Analytical Ultracentrifugation

• Sedimentation Velocity.
  • Sedimentation coefficient.
  • Diffusion coefficient (if the sedimenting components are well separated).
  • Effective mass of solute components.
  • Shape information Molecular weight.
• Sedimentation Equilibrium.
  • Molecular weight.
  • Homogeneity with respect to molecular weight.
  • Aggregation states.
  • Stoichiometry and equilibrium constants for association processes.
• More Reference Publications.

• XL-A, Beckman Coulter, 1-800-551-1150
• Rotor: The An-60 Ti is a 4-place titanium rotor rated for 60,000 rpm.

• 1995

• Please visit Beckman's web site for more information.
• Refer to the XL-A Operating Procedure (not yet available) for more information about exact absorbance.
  • Sedimentation Velocity.
    • Sample: At least 500 ul with 0.7-0.8 OD at scanning wavelength.
    • Buffer: At least 500 ul.
  • Sedimentation Equilibrium.
    • Sample: At least 500 ul with 0.2-0.3 OD at scanning wavelength.
    • Buffer: At least 10 ml.

• Contact facility staff to schedule a training session.
• Download the Cell Assembly Diagram (Acrobat pdf 47 kb).
• Download the XL-A Cell Assembly Procedure (Acrobat pdf 920 kb).
• Find more at the Protein Interaction Facility, University of Utah HSC.
• Reversible Associations in Structural and Molecular Biology (RASMB). The website at the Boston Biomedical Research Institute offers archived software, tutorials for time derivative and Nonlin software, bibliographies, links, and FAQs.

• Complete a Sample Submission Form if you want us to run your sample.