Differential Scanning Calorimetry Instrumentation at the Biophysics Core Facility: The Program In Biomolecular Structure

Differential Scanning Calorimetry

Applications	Conformational energy in macromolecules. Typical application areas for DSC include the following: Formulation stability studies Determination of macromolecule domain structure Chemical half-life determinations Determining thermal stability and reversibility Link to MicroCal for more information.
Instrument	Microcal. 1-800-633-3115 Technical Specifications
Date Installed	January, 2001
Typical Sample	Instrument requires 0.585 ml. You will need more than that to fill the instrument. Peptide coil-coil, Yu et al., 1999, J. Phys Chem B 103:2270. 70 amino acids total, 60 - 350 uM 35 amino acids total, 25 - 900 uM
Training	1-day self guided tutorial. Download the tutorial (Acrobat pdf 300 kb). Please read the material at the Microcal site. More information and tutorials can be found at the following sites. University of Southern Mississippi, Department of Polymer Science. http://www.psrc.usm.edu/macrog/lab/dsc.htm http://www.bindingconstants.com/ITC_DSC/ DSC_basics.htm http://www.friedli.com/research/PhD/DSC/chap3.html Science Resources, Inc.
Sample Submission	Complete a Sample Submission Form if you want us to run your sample.

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