Biacore

Recommended Reading

• Methods Enzymol 1998;295:268-94
  Kinetic analysis of macromolecular interactions using surface plasmon resonance biosensors.
  Morton TA, Myszka DG
  Division of Biochemistry and Molecular Biology, John Curtin School of Medical Research, Australian National University, Canberra, Australia.
  PubMed citation.

• J Mol Recognit 1999 Nov-Dec;12(6):390-408
  Survey of the 1998 optical biosensor literature.
  Myszka DG
  University of Utah, Salt Lake City, UT 84132, USA.
  The utilization of optical biosensors to study molecular interactions continues to expand. In 1998, 384 articles relating to the use of commercial biosensors were published in 130 different journals. While significant strides in new applications and methodology were made, a majority of the biosensor literature is of rather poor quality. Basic information about experimental conditions is often not presented and many publications fail to display the experimental data, bringing into question the credibility of the results. This review provides suggestions on how to collect, analyze and report biosensor data.
  PubMed citation.

• J Mol Recognit 1999 Sep-Oct;12(5):279-84
  Improving biosensor analysis.
  Myszka DG
  Huntsman Cancer Institute, University of Utah, Salt Lake City, UT 84132, USA.
  The quality of optical biosensor data must be improved in order to characterize the mechanism and rate constants associated with molecular interactions. Many of the artifacts associated with binding data can be minimized or eliminated by designing the experiment properly, collecting data under optimum conditions and processing the data with reference surfaces. It is possible to globally fit high-quality biosensor data with simple bimolecular reaction models, which validates the technology as a biophysical tool for interaction analysis.
  PubMed citation.