Biacore Instrumetation at the Biophysics Core Facility: The Program In Biomolecular Structure

	Biacore

Applications	Biomolecular Interactions. Seek binding partners. Monitor recombinant antibody quality. Recommended reading. More information at Biacore.
Instrument	Biacore 3000 1-800-242-2599
Date Installed	September, 2000
Capabilities	Basic Principles of Biacore. FAQ.
Typical Sample	Analyte concentration in the picomolar range, 0.1 - 100 * Kd (mM), 25 to 150 ul. Immobilized material, 1-5 ug for amine coupling. Binding in mM to pM range. Read more at Biacore's site.
Training	Contact the Facility Manager to discuss and arrange training sessions. Training consists of a 1/2-day hands-on tutorial. Download the training document here (Acrobat pdf 4.7 Mb). The Facility has a copy of BIAsimulation that you may install on your computer. This program allows you to Perform dry run experiments to verify experimental data. Save on materials and time during experimental design. Use as a learning tool for BIA analysis. Contact Facility personnel to use BIAsimulation. See also the Recommended Publications list. Proteomics reading list.
Data Analysis	Protein Interaction Facility, CLAMP Software, David Myszka, Director. Macromolecular Interactions Facility, University of North Carolina.

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