John M. Stewart, Ph.D.

Professor of Biochemistry

Department of Biochemistry and Molecular Genetics
University of Colorado Health Sciences Center
Campus Box B121
4200 East Ninth Avenue
Denver, Colorado 80262

Office Phone: 303.315.7534
John.Stewart@uchsc.edu

EDUCATION/EXPERIENCE

HONORS AND AWARDS

PROFESSIONAL ACTIVITIES

Research Interests Research in my laboratory concentrates on chemistry and biology of peptides.

Chemistry: This laboratory is well-equipped to carry out synthesis of a wide variety of peptides by the solid phase method. Improvements in synthetic methods are being developed. J.M. Stewart was the designer of the first instrument for automatic peptide synthesis and collaborated with R.B. Merrifield on development of the solid-phase method, for which Merrifield won the Nobel prize. The Stewart laboratory has carried out syntheses of a wide variety of peptides during more than 3 decades of research.

Biology: Current research deals with the following interests: Development of more potent bradykinin antagonists and application of them to the pathophysiology of inflammation and cancer. Physiological roles of the neuropeptide Substance P and elucidation of new SP receptors in the central nervous system. Analogs of luteinizing hormone releasing hormone, opioid peptides, scorpion venom peptides. Study of the rules governing protein folding and the design and synthesis of new enzymes.

Selected Publications

• Book: "Solid Phase Peptide Synthesis", J.M. Stewart and J.D. Young, W.H. Freeman, San Francisco, 1969; Russian Edition, 1971. Second Edition, Pierce Chemical Co., Rockford, IL, 1984.

• Bradykinin antagonists: Present progress and future prospects. J.M. Stewart, L. Gera, E.J. York, D.C. Chan and P. Bunn. Immunopharmacology 43: 155-161, 1999. [Abstract]

• Novel bradykinin antagonist dimers for the treatment of human lung cancers. D. Chan, L. Gera, B. Helfrich, K. Helm, J. Stewart, E. Whalley and P. Bunn. Immunopharmacology 33: 201-204, 1996.

• Potent, long-acting, orally-active bradykinin antagonists for a wide range of applications. J.M. Stewart, L. Gera, D.C. Chan, E.T. Whalley, W.L. Hanson and J.S. Zuzack. Immunopharmacology 36: 167-172, 1997. [Abstract]

• NMR and CD conformational studies of bradykinin and its agonists and antagonists: Application to receptor binding. G. Kotovych, J.R. Cann, J.M.Stewart and H. Yamamoto. Biochem. Cell Biol. 76: 257-266, 1998.[Abstract]

• Neuropeptide processing in pathophysiology. J.M. Stewart and M.E. Hall. Agents and Actions 42S: 211-226, 1993.[Abstract]

  Studies on chymotrypsin-like catalysis by synthetic peptides. M.J. Corey,. Hallakova, K. Pugh and J.M. Stewart. App. Biochem. Biotechnol., 47:199-212, 1994. [Abstract]

• Helix propensities of basic amino acids increase with the length of the side-chain. S. Padmanabhan, E.J. York, J.M. Stewart and R.L. Baldwin. J. Mol. Biol. 257: 726-734, 1996. [Abstract]
  

Bibliography