Robert C. Murphy, Ph.D.
Professor of Pharmacology
Department of Pharmacology
University of Colorado at Denver and Health Sciences Center
RC1 South Tower, Room 6118
P.O. Box 6511 MS 8303, Aurora, CO 80045
Phone: (303) 724-3352
Robert.Murphy@uchsc.edu
EDUCATION/EXPERIENCE
HONORS AND AWARDS
PROFESSIONAL ACTIVITIES
Research Interests:
Research in this laboratory focuses on the basic biochemistry and pharmacological control of lipid mediators derived from both enzymatic and nonenzymatic pathways largely employing techniques of sophisticated mass spectrometry to address critical issues. The term lipid mediators is used here in the context that many of the compounds under investigation have potent and diverse biological activities that permit cells to intercommunicate with each other. For example, following exposure to reactive oxygen species new lipid molecules are formed from endogenous lipids by covalent alteration of their chemical structure and these new molecules cause cells in a tissue to respond to the oxidant stress. A major emphasis has continued to involve the use of mass spectrometry for both qualitative and quantitative investigations in this area of lipid biochemistry. Three specific areas are the current focus of activities in this laboratory and they include:
- Eicosanoid mediator biosynthesis
- Lipid MAPS
- Reactive oxygen and lipid biochemistry
For more detailed information, go to my home page –
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See my online Lipid Calculator –
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Selected Publications:
- Gijón MA, Zarini S, Murphy RC. Biosynthesis of eicosanoids and transcellular metabolism of leukotrienes in murine bone marrow cells. J Lipid Res 48:716-725 (2007).
- Folco G, Murphy RC. Eicosanoid transcellular biosynthesis: From cell-cell interactions to in vivo tissue responses. Pharmacol Rev 58:375-388 (2006).
- Zarini S, Gijon MA, Folco G, Murphy RC. Effect of arachidonic acid reacylation on leukotriene biosynthesis in human neutrophils stimulated with granulocyte-macrophage colony-stimulating factor and formyl-methionyl-leucyl-phenylalanine. J Biol Chem 281: 10134–10142 (2006).
- Murphy RC, Barkley RM, Zemski Berry K, Hankin JA, Harrison K, Johnson C, Krank J, McAnoy A, Uhlson C, Zarini S. Electrospray ionization and tandem mass spectrometry of eicosanoids. Anal Biochem 346:1-42 (2005).
- McAnoy AM, Wu CC, Murphy RC. Direct qualitative analysis of triacylglycerols by electrospray mass spectrometry using a linear ion trap. J Am Soc Mass Spectrom 16:1498-1509 (2005).
- Zemski Berry KA, Murphy RC. Analysis of cell membrane aminophospholipids as isotope-tagged derivatives. J Lipid Res 46:1038-1046 (2005).
- Pulfer MK, Taube C, Gelfand E, Murphy RC. Ozone exposure in vivo and formation of biologically active oxysterols in the lung. J Pharmacol Exp Ther 312:256-264 (2005).
- Pulfer MK, Murphy RC. Formation of biologically active oxysterols during ozonolysis of cholesterol present in lung surfactant. J Biol Chem 279:26331-26338 (2004).
- Dickinson Zimmer JS, Voelker DR, Bernlohr DA, Murphy RC. Stabilization of leukotriene A4 by epithelial fatty acid binding protein in the rat basophilic leukemia cell. J Biol Chem 279:7420-7426 (2004).
Bibliography
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